GTPase activating protein for ARF GTPAse (ARFGAP) from the malaria parasite Plasmodium falciparum was expressed, purified and crystallized. Crystals of ARFGAP belong to trigonal space group P321 (or its enantiomorph) with unit cell parameters a=b=95.89 and c=92.46 Å. Diffraction data to 2.4-Å resolution have been collected. Calculation of self-rotation function suggested the presence of two molecules in the asymmetric unit. © 2003 Elsevier B.V. All rights reserved.