Characterization of gastric mucosal membranes. Composition of gastric cell membranes and polypeptide fractionation using ionic and nonionic detergents

Academic Article

Abstract

  • Lipid, carbohydrate, amino acid, and polypeptide composition of 2 fractions of cell membranes obtained by sucrose-Ficoll zonal density gradient fractionation of gastric mucosal homogenates has been studied. The membranes with density = 1.04 contain 0.7 μmole lipid P and 0.54 μmole cholesterol/mg protein, while the membranes of density 1.10 contain only 0.25 μmole lipid P and 0.28 μmole cholesterol/mg protein. Phosphatidylcholine and phosphatidylethanolamine are the most abundant phospholipids. Free fatty acids were present. Carbohydrates were most abundant in the proteins of the Peak II membranes (density = 1.10). One polypeptide band was dominant on sodium dodecyl sulfate, Triton X-100, and Brij 36T gels, and the adenosine triphosphatase activity in Brij 36T gels was found to have a relative mobility of 0.14 and 0.19 in the two fractions. © 1974.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Spenney JG; Saccomani G; Spitzer HL; Tomana M; Sachs G
  • Start Page

  • 456
  • End Page

  • 471
  • Volume

  • 161
  • Issue

  • 2