Monitoring of Murine Osteosarcoma by Serial Alkaline Phosphatase Determinations

Academic Article


  • To establish that alkaline phosphatase (AP) was released by osteosarcoma cells, we measured this enzyme in C3H/HeJ mice with im-implanted osteosarcoma and in in vitro cultures of neoplastic cells subjected to short-term incubation. We found that 105 osteosarcoma cells synthesized a significant amount of AP in vitro in 30 minutes at 37° C. A good correlation existed between pulmonary metastatic tumors and the AP values. Serum AP measurements indicated approximate sizes of disseminated and localized tumors, but could not monitor early localized tumors. © 1976, Oxford University Press.
  • Digital Object Identifier (doi)

    Author List

  • Ghanta VK; Hiramoto RN
  • Start Page

  • 837
  • End Page

  • 839
  • Volume

  • 57
  • Issue

  • 4