Evaluation of DNA ploidy in dysplastic and Spitz nevi by flow cytometry

Academic Article


  • As many as 40% of all primary cutaneous melanomas can have histologic remnants of nevomelanocytic nevi adjacent to the tumor. There is increasing evidence that dysplastic nevi are at least a clinical marker for melanoma risk. Spitz nevi are not known for such an association, but are noteworthy because of their histologic appearances. Spitz and dysplastic nevi were studied by flow cytometry to search for DNA abnormality. The study material consisted of formalin‐fixed, paraffin embedded material of 41 dysplastic nevi and 14 Spitz nevi. Four cases of dysplastic nevi were excluded for technical reasons. Of the 37 interpretable histograms from dysplastic nevi, 28 (76%) were diploid and nine (24%) were aneuploid. All the Spitz nevi were diploid. Thus, dysplastic nevi, but not Spitz nevi, share aneu‐ploidy features in some cases with melanoma. Previous authors have demonstrated aneuploidy in mlanoma with aggressive behavior and in those in deep vertical growth phase. Aneuploidy may be a feature of early as well as late stages of tumor progression regarding the nevomelanocyte system. Copyright © 1990, Wiley Blackwell. All rights reserved
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Winokur TS; Palazzo JP; Johnson WC; Duray PH
  • Start Page

  • 342
  • End Page

  • 347
  • Volume

  • 17
  • Issue

  • 6