Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties

Academic Article

Abstract

  • We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Orihuela CJ; Janssen R; Robb CW; Watson DA; Niesel DW
  • Start Page

  • 6082
  • End Page

  • 6086
  • Volume

  • 68
  • Issue

  • 10