Polyclonal B-cell activation induced by extracts of gram-negative bacteria isolated from periodontally diseased sites

Academic Article

Abstract

  • The objective of this research was to determine whether gram-negative bacteria frequently isolated from periodontally diseased sites contained polyclonal B-cell activators. Polyclonal B-cell activation, which results in nonspecific activation of multiple B-cell clones, was analyzed by a hemolysis-in-gel assay designed to detect a broad range of antibody specificities. Extracts from numerous bacterials strains, including Bacteroides gingivalis, Bacteroides melaninogenicus subsp. melaninogenicus, B. melaninogenicus subsp. intermedius, Fusobacterium nucleatum, Selenomonas sputigena, Capnocytophaga ochracea, and Actinobacillus actinomycetemcomitans, were tested. Extracts of the above organisms were found to stimulate polyclonal antibody responses in cultures of normal human peripheral blood lymphocytes, although the magnitude of stimulation varied among the extract. Optimal antibody-forming cell responses were found at stimulator doses between 5 and 1,000 μg/ml. We conclude that the resident gram-negative subgingival flora associated with periodontal lesions possesses potent polyclonal B-cell activators. These activators may contribute to disease pathogenesis by inducing B lymphocytes to produce antibody, osteolytic factors, or both and possibly other mediators of inflammation.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Bick PH; Carpenter AB; Holdeman LV; Miller GA; Ranney RR; Palcanis KG; Tew JG
  • Start Page

  • 43
  • End Page

  • 49
  • Volume

  • 34
  • Issue

  • 1