Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene

Academic Article

Abstract

  • Leishmania parasites (order Kinetoplastida, family Trypanosomatidae) cause a spectrum of human diseases ranging from asymptomatic to lethal. The ∼33.6 Mb genome is distributed among 36 chromosome pairs that range in size from ∼0.3 to 2.8 Mb. The complete nucleotide sequence of Leishmania major Friedlin chromosome 1 revealed 79 protein-coding genes organized into two divergent polycistronic gene clusters with the mRNAs transcribed towards the telomeres. We report here the complete nucleotide sequence of chromosome 3 (384 518 bp) and an analysis revealing 95 putative protein-coding ORFs. The ORFs are primarily organized into two large convergent polycistronic gene clusters (i.e. transcribed from the telomeres). In addition, a single gene at the left end is transcribed divergently towards the telomere, and a tRNA gene separates the two convergent gene clusters. Numerous genes have been identified, including those for metabolic enzymes, kinases, transporters, ribosomal proteins, spliceosome components, helicases, an RNA-binding protein and a DNA primase subunit.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Worthey EA; Martinez-Calvillo S; Schnaufer A; Aggarwal G; Cawthra J; Fazelinia G; Fong C; Fu G; Hassebrock M; Hixson G
  • Start Page

  • 4201
  • End Page

  • 4210
  • Volume

  • 31
  • Issue

  • 14