James Kobie

James Kobie
Associate Professor

Scientist (C), UAB Immunology Institute , School of Medicine
Scientist (C), Center for AIDS Research , School of Medicine
Scientist (C), Center for Clinical and Translational Science (CCTS) , General Clinical Research Center
Associate Professor (P), Medicine - Infectious Diseases , Department of Medicine

Selected Publications

Academic Article

Year	Title	Altmetric
2023	Mixed Origins: HIV gp120-Specific Memory Develops from Pre-Existing Memory and Naive B Cells Following Vaccination in Humans.. AIDS Research and Human Retroviruses. 2023
2023	Antibodies, B Cell Responses and Immune Responses to SARS-CoV-2 Infections.. Antibodies. 12. 2023
2022	Baseline host determinants of robust human HIV-1 vaccine-induced immune responses: A meta-analysis of 26 vaccine regimens. EBioMedicine. 84. 2022
2022	Monitoring SARS-CoV-2 Infection Using a Double Reporter-Expressing Virus. Microbiology Spectrum. 10. 2022
2022	Potent universal beta-coronavirus therapeutic activity mediated by direct respiratory administration of a Spike S2 domain-specific human neutralizing monoclonal antibody. PLoS Pathogens. 18. 2022
2022	Monitoring SARS-CoV-2 infection using a double reporter-expressing virus 2022
2022	Immunization with recombinant accessory protein-deficient SARS-CoV-2 protects against lethal challenge and viral transmission. 2022
2022	Potent universal-coronavirus therapeutic activity mediated by direct respiratory administration of a Spike S2 domain-specific human neutralizing monoclonal antibody. 2022
2021	A bifluorescent-based assay for the identification of neutralizing antibodies against SARS-CoV-2 variants of concern in vitro and in vivo. Journal of Virology. 95. 2021
2021	CD4+ T Cells Are Dispensable for Induction of Broad Heterologous HIV Neutralizing Antibodies in Rhesus Macaques. Frontiers in Immunology. 12. 2021
2021	Analysis of SARS-CoV-2 infection dynamic in vivo using reporter-expressing viruses 2021
2021	Duration of post-COVID-19 symptoms is associated with sustained SARS-CoV-2-specific immune responses. JCI Insight. 6. 2021
2021	Epitope Classification and RBD Binding Properties of Neutralizing Antibodies Against SARS-CoV-2 Variants of Concern. Frontiers in Immunology. 12. 2021
2021	Generation and characterization of recombinant SARS-CoV-2 expressing reporter genes. Journal of Virology. 95. 2021
2021	Selection, identification, and characterization of SARS-CoV-2 monoclonal antibody resistant mutants. Journal of Virological Methods. 290. 2021
2021	Therapeutic activity of an inhaled potent SARS-CoV-2 neutralizing human monoclonal antibody in hamsters. Cell Reports Medicine. 2. 2021
2021	Polyfunctional tier 2-neutralizing antibodies cloned following hiv-1 env macaque immunization mirror native antibodies in a human donor. Journal of Immunology. 206:999-1012. 2021
2021	Sustained cellular immune dysregulation in individuals recovering from SARS-CoV-2 infection. Journal of Clinical Investigation. 131. 2021
2021	Rapid in vitro assays for screening neutralizing antibodies and antivirals against SARS-CoV-2. Journal of Virological Methods. 287. 2021
2020	Impact of vaccine type on HIV-1 vaccine elicited antibody durability and B cell gene signature. Scientific Reports. 10. 2020
2020	Sustained Cellular Immune Dysregulation in Individuals Recovering from SARS-CoV-2 Infection 2020
2020	Cloning and functional testing of rhesus macaque (Macaca mulatta) IL-9 and IL-33. Journal of Medical Primatology. 49:144-152. 2020
2020	Persistence of HIV-1 Env-Specific Plasmablast Lineages in Plasma Cells after Vaccination in Humans. Cell Reports Medicine. 1. 2020
2020	A broad and potent H1-specific human monoclonal antibody produced in plants prevents influenza virus infection and transmission in guinea pigs 2020
2020	A broad and potent H1-specific human monoclonal antibody produced in plants prevents influenza virus infection and transmission in Guinea pigs. Viruses. 12. 2020
2020	The Effects of Trauma, Violence, and Stress on Sexual Health Outcomes Among Female Clinic Clients in a Small Northeastern U.S. Urban Center.. Women's Health Reports. 1:132-142. 2020
2019	DNA priming and gp120 boosting induces HIV-specific antibodies in a randomized clinical trial. Journal of Clinical Investigation. 129:4769-4785. 2019
2019	Identification of variant HIV envelope proteins with enhanced affinities for precursors to anti-gp41 broadly neutralizing antibodies. PLoS One. 14. 2019
2019	IL-33 enhances the kinetics and quality of the antibody response to a DNA and protein-based HIV-1 Env vaccine. Vaccine. 37:2322-2330. 2019
2019	Broad and protective influenza B virus neuraminidase antibodies in humans after vaccination and their clonal persistence as plasma cells. mBio. 10:1-17. 2019
2018	A highly potent and broadly neutralizing H1 influenza-specific human monoclonal antibody. Scientific Reports. 8. 2018
2018	Display of the HIV envelope protein at the yeast cell surface for immunogen development. PLoS One. 13. 2018
2017	Curaxin CBL0100 blocks HIV-1 replication and reactivation through inhibition of viral transcriptional elongation. Frontiers in Microbiology. 8. 2017
2017	A novel bromodomain inhibitor reverses HIV-1 latency through specific binding with BRD4 to promote Tat and P-TEFb association. Frontiers in Microbiology. 8. 2017
2017	Pre-existing neutralizing antibody mitigates B cell dysregulation and enhances the Envspecific antibody response in SHIV-infected rhesus macaques. PLoS One. 12. 2017
2016	Increased Steady-State Memory B Cell Subsets among High-Risk Participants in an HIV Vaccine Trial. AIDS Research and Human Retroviruses. 32:1143-1148. 2016
2016	Humoral dysregulation associated with increased systemic inflammation among injection heroin users. PLoS One. 11. 2016
2016	Identification of significant B cell associations with undetected observations using a Tobit model. Statistics and its Interface. 9:79-91. 2016
2015	Platelet activation in human immunodeficiency virus type-1 patients is not altered with cocaine abuse. PLoS One. 10. 2015
2015	Functional and Molecular Characteristics of Novel and Conserved Cross-Clade HIV Envelope Specific Human Monoclonal Antibodies. Hybridoma. 34:65-72. 2015
2014	Microbubble array diffusion assay for the detection of cell secreted factors 2014
2014	Cutting edge: An antibody recognizing ancestral endogenous virus glycoproteins mediates antibody-dependent cellular cytotoxicity on HIV-1-infected cells. Journal of Immunology. 193:1544-1548. 2014
2014	Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody 2014
2013	Neutralizing polyclonal IgG present during acute infection prevents rapid disease onset in simian-human immunodeficiency virus SHIVSF162P3-infected infant rhesus macaques. Journal of Virology. 87:10447-10459. 2013
2013	Characterization of cell seeding and specific capture of B cells in microbubble well arrays. Biomedical Microdevices. 15:453-463. 2013
2013	Anti-idiotypic monobodies derived from a fibronectin scaffold. Biochemistry. 52:1802-1813. 2013
2013	9G4+ antibodies isolated from HIV-infected patients neutralize HIV-1 and have distinct autoreactivity profiles. PLoS One. 8. 2013
2012	Anti-idiotypic monobodies for immune response profiling. ImmunoMethods. 58:62-68. 2012
2012	9G4 autoreactivity is increased in HIV-infected patients and correlates with HIV broadly neutralizing serum activity. PLoS One. 7. 2012
2011	Decreased influenza-specific B cell responses in rheumatoid arthritis patients treated with anti-tumor necrosis factor. Arthritis Research and Therapy. 13. 2011
2010	Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunization. Vaccine. 28:3582-3587. 2010
2010	Human basophils express amphiregulin in response to T cell-derived IL-3. Journal of Allergy and Clinical Immunology. 126. 2010
2009	T helper cytokine patterns: Defined subsets, random expression, and external modulation. Immunologic Research. 45:173-184. 2009
2009	Human follicular lymphoma CD39⁺-infiltrating T cells contribute to adenosine-mediated T cell hyporesponsiveness. Journal of Immunology. 183:6157-6166. 2009
2009	An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform. Immunotechnology : an international journal of immunological engineering. 341:106-116. 2009
2009	Frequencies of human influenza-specific antibody secreting cells or plasmablasts post vaccination from fresh and frozen peripheral blood mononuclear cells. Immunotechnology : an international journal of immunological engineering. 340:42-47. 2009
2008	Automated analysis of two- and three-color fluorescent Elispot (Fluorospot) assays for cytokine secretion. Computer Methods and Programs in Biomedicine. 92:54-65. 2008
2007	Temporal and spatial changes of histone 3 K4 dimethylation at the IFN-γ gene during Th1 and Th2 cell differentiation. Journal of Immunology. 179:6410-6415. 2007
2006	Amphiregulin, a TH2 cytokine enhancing resistance to nematodes. Science. 314:1746. 2006
2006	T regulatory and primed uncommitted CD4 T cells express CD73, which suppresses effector CD4 T cells by converting 5′-adenosine monophosphate to adenosine. Journal of Immunology. 177:6780-6786. 2006
2006	Short-term dietary administration of celecoxib enhances the efficacy of tumor lysate-pulsed dendritic cell vaccines in treating murine breast cancer. International Journal of Cancer. 118:2220-2231. 2006
2005	CD73 and Ly-6A/E distinguish in vivo primed but uncommitted mouse CD4 T cells from type 1 or type 2 effector cells. Journal of Immunology. 175:6458-6464. 2005
2004	α-tocopheryl succinate sensitizes established tumors to vaccination with nonmatured dendritic cells 2004
2003	Immunosuppressive role of transforming growth factor beta in breast cancer. Clinical and Applied Immunology Reviews. 3:277-287. 2003
2003	Transforming growth factor β inhibits the antigen-presenting functions and antitumor activity of dendritic cell vaccines. Cancer Research. 63:1860-1864. 2003
2001	Comparative analysis of IFN-γ B7.1 and antisense TGF-β gene transfer on the tumorigenicity of a poorly immunogenic metastatic mammary carcinoma 2001
2001	Invasion and metastasis of a mammary tumor involves TGF-β signaling. International Journal of Cancer. 91:76-82. 2001
1994	Toughness response of electrodes within arc furnace cycling. Materials Evaluation. 52. 1994

Research Overview

The Kobie Laboratory is focused on translational B cell immunology. Through the development of high throughput and high resolution technologies the lab dissects the mechanisms of protective and pathogenic B cell and antibody responses to advance vaccine development and disease therapeutics, with a primary focus on HIV and influenza.