• My clinical research focuses on diagnostic tools, techniques, and approaches that aide in the diagnosis of difficult hematopathology diagnosis. Examples of my past clinical interests included included identification and confirmation of minimal leukemic blast involvement of the CSF, identification of megakaryoblasts for diagnosing acute megakaryoblastic leukemia, use of flow cytometry for minimal detection of residual acute leukemia in pediatric patients.

    My current clinical interests focus on detection of minimal/measurable residual disease in adult hematopoietic diseases in a more objective and reproducible approaches. Specifically, I am translating and validating the flow cytometry analysis approaches used in my pediatric experience to adult diseases, testing the applicability of hiearchical clustering (machine assisted) analysis, and optimizing the use of the latest flow cytometry technology (spectral flow) for adult hematopoietic diseases.

    Improving diagnostic tools have been and will be useful in multiple collaborative research endeavors with my clinical colleagues.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2023 Laboratory Aspects of Minimal / Measurable Residual Disease Testing in B-Lymphoblastic LeukemiaClinics in Laboratory Medicine.  43:115-125. 2023
    2023 Pathologic, cytogenetic, and molecular features of acute myeloid leukemia with megakaryocytic differentiation: A report from the Children's Oncology GroupPediatric Blood and Cancer2023
    2022 Clofarabine treatment of KMT2Ar infantile patients with acute lymphoblastic leukemia in St. Jude Total Therapy Study 16Blood Advances.  6:6131-6134. 2022
    2022 The 5th edition of the World Health Organization Classification of Haematolymphoid Tumours: Lymphoid NeoplasmsLeukemia.  36:1720-1748. 2022
    2022 The 5th edition of the World Health Organization Classification of Haematolymphoid Tumours: Myeloid and Histiocytic/Dendritic NeoplasmsLeukemia.  36:1703-1719. 2022
    2022 Sorafenib in Combination With Standard Chemotherapy for Children With High Allelic Ratio FLT3 /ITD+ Acute Myeloid Leukemia: A Report From the Children's Oncology Group Protocol AAML1031Journal of Clinical Oncology.  40:2023-2035. 2022
    2022 Cytarabine dose reduction in patients with low-risk acute myeloid leukemia: A report from the Children's Oncology GroupPediatric Blood and Cancer.  69. 2022
    2021 Clinicopathologic and prognostic features of TdT-negative pediatric B-lymphoblastic leukemiaModern Pathology.  34:2050-2054. 2021
    2021 Laboratory Aspects of Minimal / Measurable Residual Disease Testing in B-Lymphoblastic LeukemiaClinics in Laboratory Medicine.  41:485-495. 2021
    2021 Clinical significance of novel subtypes of acute lymphoblastic leukemia in the context of minimal residual disease-directed therapy 2021
    2021 Integrative network analysis reveals USP7 haploinsufficiency inhibits E-protein activity in pediatric T-lineage acute lymphoblastic leukemia (T-ALL).Scientific Reports.  11:5154. 2021
    2020 Acute erythroid leukemia is enriched in NUP98 fusions: A report from the Children's Oncology GroupBlood Advances.  4:6000-6008. 2020
    2020 Integrative genomic analysis of B-lymphoblastic lymphoma with intrachromosomal amplification of chromosome 21Pediatric Blood and Cancer.  67. 2020
    2019 Clofarabine can replace anthracyclines and etoposide in remission induction therapy for childhood acute myeloid leukemia: The AML08 multicenter, randomized phase III trialJournal of Clinical Oncology.  37:2072-2081. 2019
    2019 No evidence that G6PD deficiency affects the efficacy or safety of daunorubicin in acute lymphoblastic leukemia induction therapyPediatric Blood and Cancer.  66. 2019
    2019 Genomic subtyping and therapeutic targeting of acute erythroleukemiaNature Genetics.  51:694-704. 2019
    2019 Genome-wide discovery of somatic coding and noncoding mutations in pediatric endemic and sporadic Burkitt lymphomaBlood.  133:1313-1324. 2019
    2019 Improved CNS control of childhood acute lymphoblastic leukemia without cranial irradiation: St Jude Total Therapy Study 16Journal of Clinical Oncology.  37:3377-3391. 2019
    2019 Peripheral blood flow cytometry for the diagnosis of pediatric acute leukemia: Highly reliable with rare exceptionsPediatric Blood and Cancer.  66. 2019
    2019 Significance of CNS 2 cerebrospinal fluid status post-induction in pediatric and adolescent patients with acute lymphoblastic leukemiaPediatric Blood and Cancer.  66. 2019
    2019 Treatment response and outcome of children with T-cell acute lymphoblastic leukemia expressing the gamma-delta T-cell receptorOncoImmunology.  8. 2019
    2018 Mixed-phenotype acute leukemia, T/megakaryoblasticBlood.  132:2418. 2018
    2018 The comparative sensitivity of immunohistochemical markers of megakaryocytic differentiation in acute megakaryoblastic leukemiaAmerican Journal of Clinical Pathology.  150:461-467. 2018
    2018 International cooperative study identifies treatment strategy in childhood ambiguous lineage leukemiaBlood.  132:264-276. 2018
    2018 NUP98-BPTF gene fusion identified in primary refractory acute megakaryoblastic leukemia of infancy 2018
    2018 Selective T-cell depletion targeting CD45RA reduces viremia and enhances early T-cell recovery compared with CD3-targeted T-cell depletionTransplant Infectious Disease.  20. 2018
    2018 The genetic basis and cell of origin of mixed phenotype acute leukaemiaNature.  562:373-406. 2018
    2017 CD30 expression in pediatric neoplasms, study of 585 cases 2017
    2017 BCL-W has a fundamental role in B cell survival and lymphomagenesisJournal of Clinical Investigation.  127:635-650. 2017
    2017 γδ T-Cell Acute Lymphoblastic Leukemia/Lymphoma: Discussion of Two Pediatric Cases and Its Distinction from Other Mature γδ T-Cell Malignancies.Case Reports in Hematology.  2017:5873015. 2017
    2016 Prognostic significance of major histocompatibility complex class II expression in pediatric adrenocortical tumors: A St. Jude and children's oncology group studyClinical Cancer Research.  22:6247-6255. 2016
    2016 Phase I study of selinexor, a selective inhibitor of nuclear export, in combination with fludarabine and cytarabine, in pediatric relapsed or refractory acute leukemiaJournal of Clinical Oncology.  34:4094-4101. 2016
    2016 Adult T-cell leukemia/lymphoma.Revista da Associação Médica Brasileira.  62:691-700. 2016
    2016 Metachronous T-Lymphoblastic Lymphoma and Burkitt Lymphoma in a Child With Constitutional Mismatch Repair Deficiency SyndromePediatric Blood and Cancer.  63:1454-1456. 2016
    2016 LNK/SH2B3 regulates IL-7 receptor signaling in normal and malignant B-progenitorsJournal of Clinical Investigation.  126:1267-1281. 2016
    2016 Diverse and targetable kinase alterations drive histiocytic neoplasmsCancer Discovery.  6:154-165. 2016
    2015 Outcome of children with hypodiploid ALL treated with risk-directed therapy based on MRD levelsBlood.  126:2896-2899. 2015
    2015 Atypical cutaneous γδ T cell proliferation with morphologic features of lymphoma but with clinical features and course of PLEVA or lymphomatoid papulosisJournal of Cutaneous Pathology.  42:1012-1017. 2015
    2015 The role of FDG-PET/CT in the evaluation of residual disease in paediatric non-Hodgkin lymphomaBritish Journal of Haematology.  168:845-853. 2015
    2015 Prognostic factors in children with acute myeloid leukaemia and excellent response to remission induction therapyBritish Journal of Haematology.  168:94-101. 2015
    2014 Loss of PIKfyve in platelets causes a lysosomal disease leading to inflammation and thrombosis in miceNature Communications.  5. 2014
    2014 Trisomy 21-associated transient abnormal myelopoiesis involving the maternal space of the placenta: A case report and literature review 2014
    2014 Cutaneous extranodal natural killer/T-cell lymphoma: a comparative clinicohistopathologic and survival outcome analysis of 45 cases according to the primary tumor site.Journal of the American Academy of Dermatology.  70:1002-1009. 2014
    2014 Impact of tyrosine kinase inhibitors on minimal residual disease and outcome in childhood Philadelphia chromosome-positive acute lymphoblastic leukemiaCancer.  120:1514-1519. 2014
    2014 Relapsed nodular sclerosis hodgkin lymphoma and therapy-related myeloid sarcoma in a mediastinal massBlood.  123:1291. 2014
    2014 Adult T-cell leukemia/lymphomaArchives of Pathology and Laboratory Medicine.  138:282-286. 2014
    2014 Histologic findings in skin biopsy in a JMML rash: A case report and review of literature 2014
    2014 Phase I Study of the Safety and Pharmacokinetics of Plerixafor in Children Undergoing a Second Allogeneic Hematopoietic Stem Cell Transplantation for Relapsed or Refractory LeukemiaBiology of Blood and Marrow Transplantation.  20:1224-1228. 2014
    2013 RhoA Is Essential for Maintaining Normal Megakaryocyte Ploidy and Platelet GenerationPLoS One.  8. 2013
    2013 Patient-derived induced pluripotent stem cells recapitulate hematopoietic abnormalities of juvenile myelomonocytic leukemiaBlood.  121:4925-4929. 2013
    2013 Intestinal γδ T-cell lymphomas are most frequently of type II enteropathy-associated T-cell typeHuman Pathology.  44:1131-1145. 2013
    2013 Pediatric MLBL: Challenges remainBlood.  121:245-246. 2013
    2013 CD30 expression defines a novel subgroup of diffuse large B-cell lymphoma with favorable prognosis and distinct gene expression signature: A report from the International DLBCL Rituximab-CHOP Consortium Program StudyBlood.  121:2715-2724. 2013
    2013 Platelets lacking PIP5KIγ have normal integrin activation but impaired cytoskeletal-membrane integrity and adhesionBlood.  121:2743-2752. 2013
    2012 Trisomy 21-associated defects in human primitive hematopoiesis revealed through induced pluripotent stem cells 2012
    2012 α-Hemoglobin-stabilizing protein is a sensitive and specific marker of erythroid precursorsAmerican Journal of Surgical Pathology.  36:1538-1547. 2012
    2012 Detection of leukemic lymphoblasts in CSF is instrument-dependentAmerican Journal of Clinical Pathology.  137:795-799. 2012
    2012 Self-renewing endodermal progenitor lines generated from human pluripotent stem cellsCell Stem Cell.  10:371-384. 2012
    2012 Extranodal NK/T-cell lymphoma, nasal type, includes cases of natural killer cell and αβ, γδ, and αβ/γδ T-cell origin: A comprehensive clinicopathologic and phenotypic studyAmerican Journal of Surgical Pathology.  36:481-499. 2012
    2012 G protein βγ-subunit signaling mediates airway hyperresponsiveness and inflammation in allergic asthmaPLoS One.  7. 2012
    2010 MicroRNA expression in maturing murine megakaryocytesBlood.  116:128-138. 2010
    2010 Ezrin is highly expressed in early thymocytes, but dispensable for T cell development in micePLoS One.  5. 2010
    2010 miR-451 protects against erythroid oxidant stress by repressing 14-3-3ζGenes and Development.  24:1620-1633. 2010
    2010 NuRD mediates activating and repressive functions of GATA-1 and FOG-1 during blood development 2010
    2009 Lymphocytic vasculitis involving the central nervous system occurs in patients with X-linked lymphoproliferative disease in the absence of Epstein-Barr virus infectionPediatric Blood and Cancer.  53:1120-1123. 2009
    2009 ALK expression in rhabdomyosarcomas: Correlation with histologic subtype and fusion status 2009
    2009 Percentage of γδ T cells in panniculitis by paraffin immunohistochemical analysisAmerican Journal of Clinical Pathology.  131:820-826. 2009
    2009 Loss of pleckstrin defines a novel pathway for PKC-mediated exocytosisBlood.  113:3577-3584. 2009
    2009 Treatment with sirolimus results in complete responses in patients with autoimmune lymphoproliferative syndromeBritish Journal of Haematology.  145:101-106. 2009
    2009 Loss of PIP5KIβ demonstrates that PIP5KI isoform-specific PIP 2 synthesis is required for IP3 formation (Proceedings of the National Academy of Sciences of the United States of America (2008) 105, 37, (14064-14069) DOI: 10.1073/pnas.0804139105) 2009
    2009 A robust xenotransplantation model for acute myeloid leukemiaLeukemia.  23:2109-2117. 2009
    2008 Trisomy 21 enhances human fetal erythro-megakaryocytic developmentBlood.  112:4503-4506. 2008
    2008 Loss of PIP5KIβ demonstrates that PIP5KI isoform-specific PIP 2 synthesis is required for IP3 formation 2008
    2008 γc-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytesJournal of Immunology.  181:2506-2512. 2008
    2008 Lethal vascular leak syndrome after denileukin diftitox administration to a patient with cutaneous gamma/delta T-cell lymphoma and occult cirrhosisAmerican Journal of Hematology.  83:593-595. 2008
    2008 Prospective tracing of MLL-FRYL clone with low MEIS1 expression from emergence during neuroblastoma treatment to diagnosis of myelodysplastic syndromeBlood.  111:3802-3812. 2008
    2008 Targeting Notch signaling in autoimmune and lymphoproliferative diseaseBlood.  111:705-714. 2008
    2007 Phospholipase Cβ is critical for T cell chemotaxisJournal of Immunology.  179:2223-2227. 2007
    2007 An erythroid chaperone that facilitates folding of α-globin subunits for hemoglobin synthesisJournal of Clinical Investigation.  117:1856-1865. 2007
    2007 A potential screening tool for IPEX syndrome 2007
    2007 Mediastinal adenopathy, lung infiltrates, and hemophagocytosis: Unusual manifestation of pediatric anaplastic large cell lymphoma: Report of two casesAmerican Journal of Clinical Pathology.  127:458-464. 2007
    2007 Pediatric primary bone lymphoma-diffuse large B-cell lymphoma: Morphologic and immunohistochemical characteristics of 10 casesAmerican Journal of Clinical Pathology.  127:47-54. 2007
    2006 Rapamycin improves lymphoproliferative disease in murine autoimmune lymphoproliferative syndrome (ALPS)Blood.  108:1965-1971. 2006
    2006 The mTOR inhibitor CCI-779 induces apoptosis and inhibits growth in preclinical models of primary adult human ALLBlood.  107:1149-1155. 2006
    2006 Primary non-Hodgkin's lymphoma of bone in children 2006
    2005 Constitutively active Notch1 induces growth arrest of HPV-positive cervical cancer cells via separate signaling pathwaysExperimental Cell Research.  305:343-354. 2005
    2005 Early molecular detection of central nervous system relapse in a child with systemic anaplastic large cell lymphoma: Case report and review of the literaturePediatric Blood and Cancer.  44:400-406. 2005
    2005 Unmasking Evans syndrome: T-cell phenotype and apoptotic response reveal autoimmune lymphoproliferative syndrome (ALPS)Blood.  105:2443-2448. 2005
    2004 Identification of cyclin D3 as a direct target of E2A using DamIDMolecular and Cellular Biology.  24:8790-8802. 2004
    2004 Neuromedin U: A Myb-regulated autocrine growth factor for human myeloid leukemiasBlood.  104:1833-1840. 2004
    2004 Pulmonary hypertension complicating bone marrow transplantation for idiopathic myelofibrosisJournal of Pediatric Hematology/Oncology.  26:393-397. 2004
    2004 Tie2-Cre-Induced Inactivation of a Conditional Mutant Nf1 Allele in Mouse Results in a Myeloproliferative Disorder that Models Juvenile Myelomonocytic LeukemiaPediatric Research.  55:581-584. 2004
    2004 Cell division rates of primary human precursor B cells in culture reflect in vivo ratesStem Cells.  22:1111-1120. 2004
    2004 Immunohistochemical Detection of Aspergillus Species in Pediatric Tissue SamplesAmerican Journal of Clinical Pathology.  121:18-25. 2004
    2004 Immunohistochemical detection of Aspergillus species in pediatric tissue samples.American Journal of Clinical Pathology.  121:18-25. 2004
    2002 The coiled-coil domain and Tyr177 of bcr are required to induce a murine chronic myelogenous leukemia-like disease by bcr/ablBlood.  99:2957-2968. 2002
    2001 Promotion of cell cycle progression by basic helix-loop-helix E2AMolecular and Cellular Biology.  21:6346-6357. 2001
    2001 Hybrid HIV/MSCV LTR enhances transgene expression of lentiviral vectors in human CD34+ hematopoietic cellsStem Cells.  19:236-246. 2001
    1999 Hodgkin's disease presenting as a submental massJournal of oral surgery, anesthesia, and hospital dental service.  57:1363-1366. 1999
    1998 Subcutaneous panniculitis-like T-cell lymphoma. Clinicopathologic, immunophenotypic, and genotypic analysis of alpha/beta and gamma/delta subtypesAmerican Journal of Surgical Pathology.  22:881-893. 1998
    1998 Dispensability of the actin-binding site and spectrin repeats for targeting sarcomeric α-actinin into maturing Z bands in vivo: Implications for in vitro binding studiesDevelopmental Biology.  199:291-308. 1998
    1998 Expression of constitutively active Raf-1 in the mitochondria restores antiapoptotic and leukemogenic potential of a transformation-deficient BCR/ABL mutantJournal of Experimental Medicine.  187:1995-2007. 1998
    1997 Transformation of hematopoietic cells by BCR/ABL requires activation of a PI-3k/Akt-dependent pathway 1997
    1997 Unanticipated temporal and spatial effects of sarcomeric α-actinin peptides expressed in PtK2 cellsCytoskeleton.  38:54-74. 1997
    1997 Decreased immunoglobulin deposition in tumors and increased immature B cells in p53-null miceMolecular Cancer Research.  8:121-131. 1997
    1996 E47 activates the Ig-heavy chain and TdT loci in non-B cells 1996
    1995 Increasing intracellular concentrations of thymosin β4 in PtK2 cells: Effects on stress fibers, cytokinesis, and cell spreadingCytoskeleton.  31:307-322. 1995
    1994 Sequential appearance of muscle‐specific proteins in myoblasts as a function of time after cell division: Evidence for a conserved myoblast differentiation program in skeletal muscleCytoskeleton.  29:1-19. 1994
    1992 A sarcomeric α-actinin truncated at the carboxyl end induces the breakdown of stress fibers in PtK2 cells and the formation of nemaline-like bodies and breakdown of myofibrils in myotubes 1992
    1992 The vinculin/sarcomeric-α-actinin/α-actin nexus in cultured cardiac myocytes 1992
    1992 G-actin pool and actin messenger RNA during development of the apical processes of the retinal pigment epithelial cells of the chickDevelopmental Biology.  149:239-246. 1992
    1991 Phorbol esters selectively and reversibly inhibit a subset of myofibrillar genes responsible for the ongoing differentiation program of chick skeletal myotubesMolecular and Cellular Biology.  11:4473-4482. 1991
    1990 Autonomous Expression of the Differentiation Programs of Cells in the Cardiac and Skeletal Myogenic LineagesAnnals of the New York Academy of Sciences.  599:158-169. 1990
    1990 MyoD converts primary dermal fibroblasts, chondroblasts, smooth muscle, and retinal pigmented epithelial cells into striated mononucleated myoblasts and multinucleated myotubes 1990
    1983 Purification of protein methylase II from human erythrocytesJournal of Proteomics.  8:9-14. 1983
    1981 Microsome-dependent methylation of erythrocyte proteins by dimethylnitrosamineCarcinogenesis.  2:179-182. 1981
    1981 In vitro methylation of human erythrocyte proteins by dimethylnitrosamine 1981


    Year Title Altmetric
    2020 Acute Myeloid Leukemia and Myelodysplastic Syndromes 2020
    2018 Hematolymphoid System.  89-136. 2018
    2018 15 Precursor Lymphoid Neoplasms.  467-480.e1. 2018
    2018 16 Acute Undifferentiated Leukemia and Mixed-Phenotype Acute Leukemias.  481-487.e1. 2018
    2018 Acute Undifferentiated Leukemia and Mixed-Phenotype Acute Leukemias.  481-487.e1. 2018
    2018 Precursor Lymphoid Neoplasms.  467-480.e1. 2018
    2015 Hodgkin Lymphoma 2015
    2015 Perinatal hematology.  257-274. 2015
    2014 Embryonic and Fetal Red Blood Cell Development 2014
    2014 Pathogenesis of Pediatric Acute Lymphoblastic Leukemias 2014
    2014 Embryonic and Fetal Red Blood Cell Development.  2352-2357. 2014
    2014 Pathogenesis of Pediatric Acute Lymphoblastic Leukemias.  1749-1758. 2014
    2012 Blackfan Diamond Anemia 2012
    2012 Chediak Higashi syndrome 2012
    2012 Hereditary Pyropoikilocytosis 2012
    2012 Transient Abnormal Myelopoiesis 2012
    2011 Hematologic abnormalities in individuals with trisomy 21 2011
    2011 Myelodysplastic/myeloproliferative disorders 2011
    2006 Blood and marrow morphology 2006
    1989 Founder cells for the cardiac and skeletal myogenic lineages 1989

    Research Overview

  • I have published over 100 peer reviewed articles and 12 book chapters with two main research focuses. One is clinically based and applies new approaches to diagnose difficult pathologic entities. The other is more bench science based and focuses on increasing our understanding of the transcription factor TCF3 and its relationship to normal and leukemic B cell development.
  • Education And Training

  • University of Pennsylvania, Postdoctoral Research
  • Hospital of the University of Pennsylvania, Residency
  • Hospital of the University of Pennsylvania, Postdoctoral Fellowship
  • Doctor of Philosophy in Biochemistry, University of Pennsylvania 1991
  • Doctor of Medicine, University of Pennsylvania 1991
  • Bachelor of Science or Mathematics in Biochemistry, Cornell University 1982
  • Full Name

  • John Choi