James Collawn Ph.D.

James Collawn Ph.D.
Professor

Senior Scientist, Inflammation, Immunology and Immunotherapeutics , O'Neal Comprehensive Cancer Center
Professor (P), Cell, Developmental and Integrative Biology (CDIB) , Academic Joint Departments

Overview

James F. Collawn, Professor of Cell Biology, received his B.S. degree from the University of South Carolina and his Ph.D. degree in Biochemistry from the Medical University of South Carolina (1986). He completed his postdoctoral training in cellular immunology at The Scripps Research Institute and in cell biology at The Salk Institute in La Jolla, California. After working for two years as a Staff Scientist at The Salk Institute, Dr. Collawn joined the Department of Cell Biology in 1993. Dr. Collawn was an Established Investigator of the American Heart Association and Director of the Cell Biology Graduate Program from 2000 to 2017. He was an Associate Editor for the American Journal of Physiology-Lung Cellular and Molecular Physiology from 2011 to 2018 and is Vice-chairman of the Department of Cell, Developmental and Integrative Biology.

Selected Publications

Academic Article

Year	Title	Altmetric
2023	A kidney resident macrophage subset is a candidate biomarker for renal cystic disease in preclinical models. Disease Models and Mechanisms. 16. 2023
2022	Red blood cell exosome hemoglobin content increases after cardiopulmonary bypass and mediates acute kidney injury in an animal model. Journal of Thoracic and Cardiovascular Surgery. 164:e289-e308. 2022
2022	The transition from HIF-1 to HIF-2 during prolonged hypoxia results from reactivation of PHDs and HIF1A mRNA instability 2022
2022	The Role of the Hypoxia-Related Unfolded Protein Response (UPR) in the Tumor Microenvironment. Cancers. 14. 2022
2022	The hypoxia-induced changes in miRNA-mRNA in RNA-induced silencing complexes and HIF-2 induced miRNAs in human endothelial cells 2022
2022	HYPOXIA-INDUCIBLE FACTOR (HIF)-3α2 SERVES AS AN ENDOTHELIAL CELL FATE EXECUTOR DURING CHRONIC HYPOXIA. EXCLI Journal : Experimental and Clinical Sciences. 21:454-469. 2022
2022	Plasma Exosome Hemoglobin Released During Surgery Is Associated With Cardiac Injury in Animal Model. Annals of Thoracic Surgery. 2022
2021	Molecularly targeted nanoparticles: an emerging tool for evaluation of expression of the receptor for advanced glycation end products in a murine model of peripheral artery disease 2021
2021	Triazoloacridone C-1305 impairs XBP1 splicing by acting as a potential IRE1α endoribonuclease inhibitor 2021
2021	Genome-wide mRNA profiling identifies X-box-binding protein 1 (XBP1) as an IRE1 and PUMA repressor. Cellular and Molecular Life Sciences. 78:7061-7080. 2021
2020	The effects of single nucleotide polymorphisms in cancer rnai therapies. Cancers. 12:1-20. 2020
2020	Genome-wide mRNA profiling identifies RCAN1 and GADD45A as regulators of the transitional switch from survival to apoptosis during ER stress 2020
2020	Utilizing genome-wide mrna profiling to identify the cytotoxic chemotherapeutic mechanism of triazoloacridone C-1305 as direct microtubule stabilization. Cancers. 12. 2020
2020	Unfolded protein response (UPR) integrated signaling networks determine cell fate during hypoxia 2020
2020	Reduced Left Atrial Emptying Fraction and Chymase Activation in Pathophysiology of Primary Mitral Regurgitation. JACC: Basic to Translational Science. 5:109-122. 2020
2020	IRE1 endoribonuclease activity modulates hypoxic hif-1α signaling in human endothelial cells. Biomolecules. 10:1-14. 2020
2020	SARS-CoV-2 may regulate cellular responses through depletion of specific host miRNAs 2020
2019	Editorial focus: Understanding off-target effects as the key to successful RNAi therapy 2019
2019	Activation of the Human Angiotensin-(1-12)-Chymase Pathway in Rats With Human Angiotensinogen Gene Transcripts. Frontiers in Cardiovascular Medicine. 6. 2019
2019	Plasma xanthine oxidase activity is related to increased sodium and left ventricular hypertrophy in resistant hypertension. Free Radical Biology and Medicine. 134:343-349. 2019
2019	miRNA networks modulate human endothelial cell adaptation to cyclic hypoxia. Cellular Signalling. 54:150-160. 2019
2019	MiR-34c-5p modulates X-box-binding protein 1 (XBP1) expression during the adaptive phase of the unfolded protein response 2019
2019	Primary endothelial-specific regulation of hypoxiainducible factor (HIF)-1 and HIF-2 and their target gene expression profiles during hypoxia 2019
2018	eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells. Angiogenesis. 21:711-724. 2018
2018	miRNAs regulate the HIF switch during hypoxia: a novel therapeutic target. Angiogenesis. 21:183-202. 2018
2018	Therapeutic attenuation of the epithelial sodium channel with a SPLUNC1-derived peptide in airway diseases 2018
2018	microRNA single polynucleotide polymorphism influences on microRNA biogenesis and mRNA target specificity. Gene. 640:66-72. 2018
2018	Multifunctional role of chymase in acute and chronic tissue injury and remodeling. Circulation Research. 122:319-336. 2018
2017	miR-200b downregulates Kruppel Like Factor 2 (KLF2) during acute hypoxia in human endothelial cells. European Journal of Cell Biology. 96:758-766. 2017
2017	Chloroplast PetD protein: Evidence for SRP/Alb3-dependent insertion into the thylakoid membrane. BMC Plant Biology. 17. 2017
2017	miR-200b downregulates CFTR during hypoxia in human lung epithelial cells 2017
2017	Influenza virus infection alters ion channel function of airway and alveolar cells: Mechanisms and physiological sequelae 2017
2017	Ion channels of the lung and their role in disease pathogenesis 2017
2017	The therapeutic potential of CFTR modulators for COPD and other airway diseases. Current Opinion in Pharmacology. 34:132-139. 2017
2017	Desmin loss and mitochondrial damage precede left ventricular systolic failure in volume overload heart failure 2017
2017	SNPs in microRNA target sites and their potential role in human disease. Open Biology. 7. 2017
2016	Codon bias and the folding dynamics of the cystic fibrosis transmembrane conductance regulator 2016
2016	Disruption of desmin-mitochondrial architecture in patients with regurgitant mitral valves and preserved ventricular function. Journal of Thoracic and Cardiovascular Surgery. 152:1059-1070.e2. 2016
2016	Primacy of cardiac chymase over angiotensin converting enzyme as an angiotensin-(1-12) metabolizing enzyme. Biochemical and Biophysical Research Communications. 478:559-564. 2016
2016	Analysis of cystic fibrosis–associated P67L CFTR illustrates barriers to personalized therapeutics for orphan diseases. JCI Insight. 1. 2016
2016	Posttranscriptional and transcriptional regulation of endothelial nitric-oxide synthase during hypoxia: The role of microRNAs 2016
2016	Reuse of E-plate cell sensor arrays in the xCELLigence real-time cell analyzer 2016
2016	Intracrine angiotensin ii functions originate from noncanonical pathways in the human heart 2016
2016	Increased fibroblast chymase production mediates procollagen autophagic digestion in volume overload. Journal of Molecular and Cellular Cardiology. 92:1-9. 2016
2015	Volume overload induces autophagic degradation of procollagen in cardiac fibroblasts. Journal of Molecular and Cellular Cardiology. 89:241-250. 2015
2015	Concurrent DNA Copy-Number Alterations and Mutations in Genes Related to Maintenance of Genome Stability in Uninvolved Mammary Glandular Tissue from Breast Cancer Patients. Human Mutation. 36:1088-1099. 2015
2015	▵F508 CFTR surface stability is regulated by DAB2 and CHIP-mediated ubiquitination in post-endocytic compartments. PLoS One. 10. 2015
2015	The hypoxia-inducible miR-429 regulates hypoxia-inducible factor-1α expression in human endothelial cells through a negative feedback loop 2015
2015	Cardiomyocyte mitochondrial oxidative stress and cytoskeletal breakdown in the heart with a primary volume overload 2015
2015	Cardiomyocyte mitochondrial oxidative stress and cytoskeletal breakdown in the heart with a primary volume overload 2015
2015	Role of epithelial sodium channels in the regulation of lung fluid homeostasis 2015
2014	CFTR and lung homeostasis 2014
2014	Rescuing ΔF508 CFTR with trimethylangelicin, A dual-acting corrector and potentiator 2014
2014	Negative regulation of NADPH oxidase 4 by Hydrogen peroxide-inducible clone 5 (Hic-5) protein. Journal of Biological Chemistry. 289:18270-18278. 2014
2014	RNAdigest: A web-based tool for the analysis and prediction of structure - Specific RNAse digestion results. PLoS One. 9. 2014
2014	Chymase mediates injury and mitochondrial damage in cardiomyocytes during acute ischemia/reperfusion in the dog. PLoS One. 9. 2014
2013	The silent codon change I507-ATC→ATT contributes to the severity of the AF508 CFTR channel dysfunction 2013
2013	Cigarette smoke and CFTR: Implications in the pathogenesis of COPD 2013
2013	Influenza matrix protein 2 alters CFTR expression and function through its ion channel activity 2013
2013	Regulation of angiogenesis by hypoxia: The role of microRNA 2013
2013	Regulation of the unfolded protein response by microRNAs 2013
2012	The role of CFTR in transepithelial liquid transport in pig alveolar epithelia 2012
2012	The CFTR and ENaC debate: How important is ENaC in CF lung disease? 2012
2012	Glioma-specific cation conductance regulates migration and cell cycle progression. Journal of Biological Chemistry. 287:4053-4065. 2012
2012	Dab2 is a key regulator of endocytosis and post-endocytic trafficking of the cystic fibrosis transmembrane conductance regulator. Biochemical Journal. 441:633-643. 2012
2011	The Unfolded Protein Response (UPR)-activated transcription factor X-box-binding protein 1 (XBP1) induces microRNA-346 expression that targets the human antigen peptide transporter 1 (TAP1) mRNA and governs immune regulatory genes. Journal of Biological Chemistry. 286:41862-41870. 2011
2011	Enhancement of alveolar epithelial sodium channel activity with decreased cystic fibrosis transmembrane conductance regulator expression in mouse lung 2011
2011	Interaction of ASIC1 and ENaC subunits in human glioma cells and rat astrocytes 2011
2011	ARFGAP1 promotes AP-2-dependent endocytosis. Nature Cell Biology. 13:559-567. 2011
2011	CFTR expression regulation by the unfolded protein response. Methods in Enzymology. 491:3-24. 2011
2010	A synonymous single nucleotide polymorphism in ΔF508 CFTR alters the secondary structure of the mRNA and the expression of the mutant protein. Journal of Biological Chemistry. 285:28741-28748. 2010
2010	Targets for cystic fibrosis therapy: Proteomic analysis and correction of mutant cystic fibrosis transmembrane conductance regulator. Expert Review of Proteomics. 7:495-506. 2010
2010	Functional stability of rescued ΔF508 cystic fibrosis transmembrane conductance regulator in airway epithelial cells. American Journal of Respiratory Cell and Molecular Biology. 42:363-372. 2010
2009	Knockdown of ASIC1 and epithelial sodium channel subunits inhibits glioblastoma whole cell current and cell migration. Journal of Biological Chemistry. 284:24526-24541. 2009
2009	Search and rescue finding ways to correct ΔF508 CFTR. American Journal of Respiratory Cell and Molecular Biology. 40:385-387. 2009
2008	Activation of the unfolded protein response by ΔF508 CFTR. American Journal of Respiratory Cell and Molecular Biology. 39:448-457. 2008
2008	Activation of the unfolded protein response by F508 cystic fibrosis transmembrane conductance regulator. American Journal of Respiratory Cell and Molecular Biology. 39:448-457. 2008
2008	Somatic mosaicism for copy number variation in differentiated human tissues. Human Mutation. 29:1118-1124. 2008
2008	DETANO and nitrated lipids increase chloride secretion across lung airway cells. American Journal of Respiratory Cell and Molecular Biology. 39:150-162. 2008
2008	The mechanism of cystic fibrosis transmembrane conductance regulator transcriptional repression during the unfolded protein response. Journal of Biological Chemistry. 283:12154-12165. 2008
2008	Enhanced cell-surface stability of rescued ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) by pharmacological chaperones. Biochemical Journal. 410:555-564. 2008
2008	Ribulose-1,5-bisphosphate carboxylase/oxygenase from thermophilic cyanobacterium Thermosynechococcus elongatus. Photosynthesis Research. 95:101-109. 2008
2007	VCP/p97 AAA-ATPase does not interact with the endogenous wild-type cystic fibrosis transmembrane conductance regulator. American Journal of Respiratory Cell and Molecular Biology. 36:706-714. 2007
2007	Endoplasmic reticulum stress and the unfolded protein response regulate genomic cystic fibrosis transmembrane conductance regulator expression 2007
2006	Unlocking the mysteries of Na⁺-K⁺-ATPase endocytosis: Phosphorylation is the key. American Journal of Respiratory Cell and Molecular Biology. 35:1-2. 2006
2006	Activators of viral gene expression in polarized epithelial monolayers identified by rapid-throughput drug screening 2006
2006	Mutations in the amino terminus of the cystic fibrosis transmembrane conductance regulator enhance endocytosis. Journal of Biological Chemistry. 281:3329-3334. 2006
2005	Failure of cAMP agonists to activate rescued ΔF508 CFTR in CFBE41o^- airway epithelial monolayers 2005
2005	Failure of Physiological Wild Type CFTR Stimulants to Activate Rescued ∆F508 CFTR in Airway Epithelial Monolayers 2005
2005	Improved gene delivery into neuroglial cells using a fiber-modified adenovirus vector. Biochemical and Biophysical Research Communications. 328:1182-1187. 2005
2004	Efficient intracellular processing of the endogenous cystic fibrosis transmembrane conductance regulator in epithelial cell lines. Journal of Biological Chemistry. 279:22578-22584. 2004
2003	Transient transfection of polarized epithelial monolayers with CFTR and reporter genes using efficacious lipids 2003
2003	Transient transfection of polarized epithelial monolayers with CFTR and reporter genes using efficacious lipids. 2003
2003	CFTR Mediates RANTES Expression In Airway Epithelia Via Participation In A Macromolecular Signaling Complex At the Membrane Surface. Molecular and Cellular Biology. 23:549-606. 2003
2003	Plasma membrane CFTR regulates RANTES expression via its C-terminal PDZ-interacting motif. Molecular and Cellular Biology. 23:594-606. 2003
2002	Ablation of internalization signals in the carboxyl-terminal tail of the cystic fibrosis transmembrane conductance regulator enhances cell surface expression. Journal of Biological Chemistry. 277:49952-49957. 2002
2002	Reactive oxygen nitrogen species decrease cystic fibrosis transmembrane conductance regulator expression and cAMP-mediated Cl^- secretion in airway epithelia. Journal of Biological Chemistry. 277:43041-43049. 2002
2002	PDZ domain interaction controls the endocytic recycling of the cystic fibrosis transmembrane conductance regulator. Journal of Biological Chemistry. 277:40099-40105. 2002
2002	Several PATCHED1 missense mutations display activity in patched1-deficient fibroblasts. Journal of Biological Chemistry. 277:33632-33640. 2002
2002	Enhancement of MHC class II-restricted responses by receptor-mediated uptake of peptide antigens. Journal of Immunology. 169:2337-2345. 2002
2002	AP2 adaptor complex-dependent internalization of CD5: Differential regulation in T and B cells. Journal of Immunology. 168:5612-5620. 2002
2002	Genetic ablation of phosphatidylinositol transfer protein function in murine embryonic stem cells. Molecular Biology of the Cell. 13:739-754. 2002
2002	Inhibition of T cell responses by transferrin-coupled competitor peptides. Immunologic Research. 26:77-85. 2002
2001	Receptor mediated uptake of peptides that bind the human transferrin receptor 2001
2001	Identification of the transferrin receptor as a novel immunoglobulin (Ig)A1 receptor and its enhanced expression on mesangial cells in Iga nephropathy. Journal of Experimental Medicine. 194:417-425. 2001
2000	Down-regulation of cell surface receptors is modulated by polar residues within the transmembrane domain. Molecular Biology of the Cell. 11:2643-2655. 2000
1999	Casein kinase II activity is required for transferrin receptor endocytosis. Journal of Biological Chemistry. 274:30550-30556. 1999
1999	Efficient endocytosis of the cystic fibrosis transmembrane conductance regulator requires a tyrosine-based signal. Journal of Biological Chemistry. 274:3602-3609. 1999
1999	Regulation of MHC class II expression in the central nervous system 1999
1998	Structural requirements for major histocompatibility complex class II invariant chain endocytosis and lysosomal targeting. Journal of Biological Chemistry. 273:20644-20652. 1998
1998	Analysis of the structural requirements for lysosomal membrane targeting using transferrin receptor chimeras. Journal of Biological Chemistry. 273:14355-14362. 1998
1998	Construction and characterization of encapsidated poliovirus replicons that express biologically active murine interleukin-2. Journal of Interferon and Cytokine Research. 18:305-313. 1998
1996	Functional analysis of human/chicken transferrin receptor chimeras indicates that the carboxy-terminal region is important for ligand binding 1996
1994	Sorting signals in the MHC class II invariant chain cytoplasmic tail and transmembrane region determine trafficking to an endocytic processing compartment 1994
1993	Ligand-induced internalization of the epidermal growth factor receptor is mediated by multiple endocytic codes analogous to the tyrosine motif found in constitutively internalized receptors. Journal of Biological Chemistry. 268:19312-19320. 1993
1993	Signal-dependent membrane protein trafficking in the endocytic pathway. Annual Review of Cell and Developmental Biology. 9:129-161. 1993
1993	YTRF is the conserved internalization signal of the transferrin receptor, and a second YTRF signal at position 31-34 enhances endocytosis. Journal of Biological Chemistry. 268:21686-21692. 1993
1992	Structural requirements for high efficiency endocytosis of the human transferrin receptor. Journal of Inorganic Biochemistry. 47:209-217. 1992
1991	Structure-Function Analysis of the Human Transferrin Receptor: Effects of Anti-Receptor Monoclonal Antibodies on Tumor Growth 1991
1991	Transplanted LDL and mannose-6-phosphate receptor internalization signals promote high-efficiency endocytosis of the transferrin receptor 1991
1990	Transferrin receptor internalization sequence YXRF implicates a tight turn as the structural recognition motif for endocytosis. Cell. 63:1061-1072. 1990
1990	Stabilization of helical structure in two 17‐residue amphipathic analogues of the C‐terminal peptide of cytochrome c. Biospectroscopy. 29:1289-1296. 1990
1989	Preparation of Antipeptide Antibodies. Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]. 2:11.14.1-11.15.1. 1989
1989	Production of Antipeptide Antibodies: Immunology.. Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]. 6:11.15.1-11.15.4. 1989
1989	Selection of an Immunogenic Peptide: Immunology.. Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]. 6:11.14.1-11.14.4. 1989
1989	An analysis of the physical properties of peptides that influence the pigeon cytochrome c specific T lymphocyte response. Molecular Immunology. 26:1069-1079. 1989
1989	Substitutions engineered by chemical synthesis at three conserved sites in mitochondrial cytochrome c. Thermodynamic and functional consequences. Journal of Biological Chemistry. 264:15199-15209. 1989
1988	Monoclonal antibodies as probes of conformational changes in protein-engineered cytochrome c. Journal of Biological Chemistry. 263:8625-8634. 1988
1987	Isolation and partial amino acid sequence of three subunit species of porcine spleen ferritin: evidence of multiple H subunits.. Molecular cell biology research communications : MCBRC. 259:105-113. 1987
1986	Evidence that H-enriched human placental ferritin is structurally similar to L-enriched ferritins of other tissues. 1986
1986	Separation of multiple ferritin subunit types by reversed-phase HPLC. Journal of Liquid Chromatography and Related Technologies. 9:1679-1688. 1986
1985	Hydrogen-tritium exchange by apoferritin and ferritin.. Molecular cell biology research communications : MCBRC. 240:242-245. 1985
1985	A comparison by HPLC of ferritin subunit types in human tissues.. Comparative Biochemistry and Physiology - B: Comparative Biochemistry. 81:901-904. 1985
1984	A chemical and physical comparison of ferritin subunit species fractionated by high-performance liquid chromatography.. Molecular cell biology research communications : MCBRC. 233:260-266. 1984
1984	The distribution of ferritin subunit types in porcine tissues.. Comparative Biochemistry and Physiology - B: Comparative Biochemistry. 78:653-656. 1984
1983	Separation by HPLC of Two Types of Subunits from Horse Spleen Ferritin. Journal of Chromatography A. 264:463-468. 1983
1983	A single column method to measure hydrogen--tritium exchange by gel chromatography.. Journal of Proteomics. 7:311-315. 1983
1982	A sensitive assay employing a solid-phase metal chelator to estimate rates of iron accumulation by ferritin.. Analytical Biochemistry. 127:105-111. 1982
1982	Purification and Macromolecular Properties of a Sialic-Acid Specific Lectin from Limax Flavus. Journal of Biological Chemistry. 257:7574-7580. 1982
1980	Behavior of Glycopolypeptides with Empirical Molecular Weight Estimation Methods. 1. In Sodium Dodecyl Sulfate. Biochemistry. 19:5734-5741. 1980
1980	Behavior of Glycopolypeptides with Empirical Molecular Weight Estimation Methods. 2. In Random Coil Producing Solvents. Biochemistry. 19:5741-5747. 1980
1978	Thin-layer gel chromatography of proteins in mild and denaturing detergents. Journal of Chromatography A. 157:227-234. 1978

Book

Year	Title	Altmetric
2008	Chapter 1 Structure and Functions of Biomembranes. Ed. 61. 2008
2003	Encyclopedia of the Neurological Sciences 2003

Chapter

Year	Title	Altmetric
2015	Regulation of Airway Lining Fluid in Health and Disease. 467-477. 2015
2008	Structure and Functions of Biomembranes. 1-21. 2008
2006	Cystic Fibrosis: Overview. 599-610. 2006